SC3: Setting Up Effective RNAi Screens: From Design to Data to Validation

Monday, September 21, 12:00-3:00 pm

The course is designed to provide in-depth information on how to go about setting up RNAi screening experiments and how to design assays for getting optimal results. The challenges working with siRNAs and shRNAs and the delivery reagents needed to get them into the appropriate cells and tissues will be discussed. The instructors will also provide their input on best practices for the execution of experiments and interpretation of results when dealing with complex biology and informatics.

Course Instructors:

Caroline Shamu, Ph.D., Director, ICCB-Longwood Screening Facility, Harvard Medical School

• Introduction to RNAi screening
• Overview of libraries, assays and equipment for screening

Scott Martin, Ph.D., Group Lead, Functional Genomics, Genentech Inc.

• Experimental assay design and set-up
• Finding the right controls, standards and protocols

John Doench, Ph.D., Research Scientist, Broad Institute of Harvard and MIT

• Considerations for pooled screens
• Complementing RNAi and CRISPR screens

Eugen Buehler, Ph.D., Group Leader, Informatics, National Center for Advancing Translational Sciences, National Institutes of Health

• Overview of off-target effects
• Strategies for eliminating/reducing off target effects

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