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RNA interference (RNAi) is being commonly used as a screening tool for identifying and validating potential drug targets, exploring unknown cellular pathways, and for performing whole-genome functional screens. The screens developed, using both small interfering RNA (siRNA) and short hairpin RNA (shRNA), are now fairly robust and sensitive and can be performed in a reliable and high-throughput fashion. The RNAi for Functional Screening conference features talks on utilizing in vitro and in vivo RNAi screens for diverse applications, choosing the right model systems and reagents, tackling RNAi delivery, off-target effects, false positives and negatives and improving quality control and data analysis.
THURSDAY, NOVEMBER 3
1:30 pm Chairperson’s Remarks
Christophe Echeverri, Ph.D., CEO & CSO, Cenix BioScience GmbH
1:40 Talk Title to be Announced
Alex Gaither, Ph.D., Research Investigator II, Developmental and Molecular Pathways, Novartis Institutes for Biomedical Research
2:10 Screening Approaches Towards Identifying Genes Associated with DNA Re-Replication in Cancer Cells
Scott Martin, Ph.D., Team Leader, RNAi Screening, NIH Chemical Genomics Center, NIH Center for Translational Therapeutics, NIH
The NIH Chemical Genomics Center has established an RNAi screening facility that performs screens in collaboration with investigators throughout the NIH intramural community. An initial genome-wide campaign involved screening for genes associated with aberrant DNA replication. Screening was conducted using libraries comprised of both pooled and individual siRNAs. Combining these approaches led to a thorough examination of genes associated with DNA replication and served as a way to compare the value of both platforms.
2:40 RNAi Screening Comes of Age: For the Love of My Target
Hakim Djaballah, Ph.D., Director, HTS Core Facility, Memorial Sloan Kettering Cancer Center
RNAi screening has offered the premise of performing several thousand simultaneous knockdowns leading to the discovery and validation of existing and novel targets. Several years on, has the technology matured enough to keep up with its premise?
3:10 Networking Refreshment Break in the Exhibit Hall with Poster Viewing
3:45 A Targeted Screen for Small Molecule Inhibitors of the Wnt/beta-catenin Pathway
Ramanuj DasGupta, Ph.D., Assistant Professor of Pharmacology and Director, RNAi Screening Facility, New York University School of Medicine/Cancer Institute
Sponsored by
4:15 A Library for microRNA Target Identification by Drug Selection
Kevin P. Forbes, Ph.D., Senior R&D Scientist, Sigma-Aldrich Corporation
To assist discovery and identification of human microRNA (miRNA) targets, we developed the MISSION® Target ID Library, a library of cDNA cloned after a dual-selection fusion protein. Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, downstream from introducing a miRNA of interest, for cDNAs containing the miRNA’s targets. Cells containing cDNA constructs targeted by the miRNA survive the second selection, and selected cDNAs can be identified by sequencing. The cDNA library was prepared from a mixture of total RNAs from multiple human tissues and cell lines to give broad coverage of the human transcriptome. Here we present preparation of the library and its use to identify targets of miR-373.
4:45 KEYNOTE PANEL: Has RNAi Screening Delivered On Its Promise?
Moderator: Christophe Echeverri, Ph.D., CEO/CSO, Cenix BioScience GmbH
Panelists: Hakim Djaballah, Ph.D., Director, HTS Core Facility, Memorial Sloan Kettering Cancer Center
Caroline Shamu, Ph.D., Director, ICCB-Longwood Screening Facility, Harvard Medical School
Alex Gaither, Ph.D., Research Investigator II, Developmental and Molecular Pathways, Novartis Institutes for Biomedical Research
Scott Martin, Ph.D., Team Leader, RNAi Screening, NIH Chemical Genomics Center, NIH Center for Translational Therapeutics, NIH
Clive Geoffrey Jackson, Ph.D., Director, Biotech Evaluations Limited
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5:45 End of Day
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